Prospective comparison of H&E, Giemsa, and Genta stains for the diagnosis of Helicobacter pylori☆,☆☆,★
Section snippets
Methods
Patients undergoing routine diagnostic upper endoscopy for any indication were eligible for this study. Patients were not included if they were undergoing endoscopy for determination of H. pylori status after a course of anti-H. pylori therapy or if they had endoscopic evidence of bleeding. The use of medications that might suppress H. pylori (i.e., bismuth, proton pump inhibitors, or antibiotics) was recorded. Patients had two biopsy specimens taken from the antrum within 3 cm of the pylorus
Routine H&E
A total of 101 patients (mean age 44 ± 1 years; 42 men and 59 women) and thus 404 biopsy specimens were included. One patient was taking omeprazole and all of her tests were negative; a second patient was taking trimethoprim-sulfamethoxazole and all of her tests were positive. No other patients were taking proton pump inhibitors, antibiotics, or bismuth. Twelve specimens were excluded from analysis of routine H&E because of missing slides or inadequate tissue, leaving 392 specimens that were
Discussion
Although histologic examination of gastric mucosal biopsy specimens is generally considered to be the gold standard for diagnosis of H. pylori, the sensitivities and specificities reported for histologic examination are variable and frequently well below 100%. Studies using more than one criteria for H. pylori diagnosis suggest sensitivities for H&E in the range of 84% to 92%,3, 4, 5 although sensitivities as low as 47% have also been reported with routine examination by unselected pathologists.
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2020, International Journal of Pediatric OtorhinolaryngologyCitation Excerpt :Sensitivity of modified giemsa varies from 64% at low HP density and 96% at high HP density. Specificity is 98–100% at both low and high HP density [57]. Culture of HP is a very arduous task [7].
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2019, Infection, Genetics and EvolutionCitation Excerpt :The first biopsy sample was collected in a small tube containing Thioglycollate-broth medium and was shipped to our microbiology laboratory within three hours for culture. The other biopsy sample was fixed in 10% formalin for histological examination and then was stained with Giemsa stain to ascertain the presence of H. pylori and the degree of inflammation and gastritis (Dixon et al., 1996; Laine et al., 1997). Based on endoscopic and histological examinations, patients were diagnosed with chronic gastritis, atrophy, peptic ulcer and DU.
Histochemistry in the diagnosis of non-neoplastic gastrointestinal disorders
2018, Seminars in Diagnostic Pathology
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From the Departments of Medicine and Pathology, University of Southern California School of Medicine, Los Angeles, California.
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Reprint requests: Loren Laine, MD, GI Division (LAC 12-137), U.S.C. School of Medicine, 2025 Zonal Ave., Los Angeles, CA 90033.
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