Basic sciencePrognostic value of proliferative activity and nuclear morphometry for progression in TaT1 urothelial cell carcinomas of the urinary bladder☆
Section snippets
Material and methods
Tissues from 195 primary consecutive UCCs, diagnosed from 1995 to 1997, were obtained by transurethral resection or biopsy. The mean patient age at the time of diagnosis was 67.6 years (range 34 to 92); all patients were white. The tissues were fixed in 4% formaldehyde, dehydrated, embedded in paraffin, and stained with hematoxylin-eosin; 4-μm sections were made. The worst differentiated area (measurement area, minimally 2 × 2 mm and maximally 5 × 5 mm) was carefully demarcated for the Ki67,
Results
Thirteen (6.7%) of the 195 patients had progression (0 [0%] of 36 low-risk, 1 [1.1%] of 85 intermediate-risk, and 12 [16.2%] of 74 high-risk patients). The median follow-up time in the nonprogression and progression groups was 53.1 months (range 31.0 to 69.6) and 12.1 months (range 2.2 to 31.4), respectively. Table II shows the single-variate progression-free survival results. Most features were significant, but the hazard ratios of the quantitative variables were higher in general than those
Comment
The results showed that quantitative proliferation features and MNA10 are stronger prognosticators than the classic clinicopathologic risk factors and confirmed previously indicated thresholds for MAI and Ki67.25, 26 It is not surprising that these thresholds were not exactly the same, because biologic variation among the patient groups may occur. Furthermore, the sampling and quantitation methods vary slightly (eg, systematic random sampling of nuclei was previously lacking, and the Ki67 and
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Cited by (25)
Objectifying grade in Ta-T1 urothelial carcinomas of the bladder using proliferative and quantitative markers: A multicentre study in 310 bladder tumors
2019, Urologic Oncology: Seminars and Original InvestigationsCitation Excerpt :Both MNA-10 and DI were determined as part of standard daily practice by a single technician. The MAI was determined according to a protocol previously described by others [13]. Briefly, mitotic figures were counted in 10 consecutive fields of vision.
Prognostic comparison of proliferation markers and World Health Organization 1973/2004 grades in urothelial carcinomas of the urinary bladder
2014, Human PathologyCitation Excerpt :The same measurement protocol was used for PPH3 in IHC-stained whole sections. The assessment of Ki-67 was performed using the computerized automated QPRODIT system (Leica, Cambridge, UK) in the least differentiated area with the subjectively highest expression of Ki-67, again as part of routine diagnostic workup [11]. Using a 2-line grid in 200 to 300 fields of vision, the percentage of Ki-67–positive tumor cells was calculated objectively.
DNA ploidy, nuclear size, proliferation index and DNA-hypomethylation in ovarian cancer
2011, Gynecologic OncologyCitation Excerpt :One of the most important reports advocating the introduction of DNA ploidy as a prognostic discriminator for patient recruitment in phase III treatment studies, was that of Kimming et al., who revealed DNA ploidy to be as powerful as or even more powerful than residual disease after primary surgery in multivariate analysis for predicting clinical outcome in advanced ovarian cancer [13]. Prognostic value of proliferative activity and nuclear morphometry in terms of the nuclear area has also been shown in patients with bladder cancer [14,15]. To the best of our knowledge, there are no investigations studying the relationship between DNA-hypomethylation and DNA ploidy status or morphometric characteristics of the nucleus in ovarian cancer cells.
P53 as a prognostic marker for bladder cancer: A meta-analysis and review
2005, Lancet OncologyCitation Excerpt :Year of publication, absence of information on inclusion criteria, and use of multivariate analysis were significantly associated with studies that found an association between P53 changes and recurrence; year of publication and sample size were the only factors independently associated with studies finding an association between P53 and progression; and only absence of information on recruitment period remained significant for studies that found an association between P53 and mortality (webtable 10). Table 2 shows the characteristics of the largest and more qualified studies,44–50 which overall did not show conclusive results. Our meta-analysis included only those estimates (hazard ratio and 95% CI) derived from Cox regression.
Computerized morphometric study of thyroid follicular carcinoma in correlation with known prognostic factors
2005, Kaohsiung Journal of Medical Sciences
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This study was supported by grant 99-87 from the Stichting Bevordering Diagnostische Morfometrie (SBDM).