Expression and antigenic characterization of the major capsid proteins of human polyomaviruses BK and JC in Saccharomyces cerevisiae
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Acknowledgments
This work was partly supported by the Lithuanian Science Foundation, grant No. 273.
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2011, Virus ResearchCitation Excerpt :Since yeast are eukaryotes, their intracellular environment is more suitable for the correct folding of eukaryotic virus proteins as compared to bacterial expression systems (Böer et al., 2007). A considerable body of data on formation of homologous and chimeric virus-like and nucleocapsid-like particles (NLPs) of various viruses in yeast has been reported to date (Hofmann et al., 1996; Sasnauskas et al., 1999; Gedvilaite et al., 2000; Hale et al., 2002; Samuel et al., 2002; Juozapaitis et al., 2007; Freivalds et al., 2006; Kucinskaite et al., 2007). The expression of recombinant N proteins with spontaneous assembly into NLPs has been demonstrated for a number of paramyxoviruses using both prokaryotic and eukaryotic systems (Spehner et al., 1991; Hummel et al., 1992; Fooks et al., 1993; Meric et al., 1994; Warnes et al., 1995; Bhella et al., 2002).