Specific Oligonucleotide Probes for in situ Detection of a Major Group of Gram-positive Bacteria with low DNA G+C Content
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2020, Scientia HorticulturaeCitation Excerpt :Randomly selected leaves from different treatments were collected and analyzed as reported by Lo Piccolo et al. (2010). The following probes were used: HGC-green for Actinobacteria [5´Rhodamine green™(NH ester) TAT AGT TAC CAC CGC CGT 3´] (Roller et al., 1994), which hybridizes with the 23S ribosomal RNA of A. agilis UMCV2, with green fluorescence (wavelength excitation and emission are 502 and 527 nm, respectively); and LGC354B-red for Firmicutes [5´Rhodamine red™(NH ester) CGG AAG ATT CCC TAC TGC 3´) (Meier et al., 1999), which hybridizes with the 16S ribosomal RNA of B. methylotrophicus M4-96, with red fluorescence (wavelength excitation and emission are 570 and 590 nm, respectively). Both probes were synthetized by Integrated DNA Technologies, Iowa, USA.