Original CommunicationMicroRNA-10b is overexpressed in pancreatic cancer, promotes its invasiveness, and correlates with a poor prognosis
Section snippets
Cell culture and RNA isolation from cultured cells
Fifteen pancreatic cancer cell lines, namely ASPC-1, BxPC-3, KP-1N, KP-2, KP-3, PANC-1, and SUIT-2 (provided by H. Iguchi, MD, National Shikoku Cancer Center, Matsuyama, Japan), MIA-PaCa2 (Japanese Cancer Resource Bank, Tokyo, Japan), Capan-1, Capan-2, H48N, HS766T, CFPAC-1 and SW1990 (American Type Culture Collection, Manassas, VA), and NORP-1 (established in our laboratory) were used. A human pancreatic ductal epithelial cell line (HPDE6-E6E7 clone 6) immortalized by transduction with the E6
miRNA gene expression profiling of pancreatic cancer cell lines and HPDE cells using TaqMan® miRNA assays
When CFPAC-1 cells were compared with HPDE cells, 39 miRNAs showed significant differences in their expression levels (31 were upregulated and 8 were downregulated; Supplementary Table 1a); see online version. When CAPAN-1 cells were compared with HPDE cells, 30 miRNAs showed significant differences in their expression levels (12 were upregulated and 18 were downregulated; Supplementary Table 1b); see online version. Among these miRNAs, there were 15 miRNAs that showed significant differences
Discussion
In the present study, we identified several miRNAs, including miR-10b, that were differently expressed in pancreatic cancer cell lines compared with HPDE cells by miRNA profiling, and confirmed that miR-10b expression was observed frequently in pancreatic cancer cells using a panel of 15 pancreatic cancer lines. Furthermore, we showed that miR-10b was overexpressed in pancreatic cancer cells relative to normal pancreatic ductal cells. We also found that miR-10b expression promoted the
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