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Expression of an Antigen Associated with Basal Bodies of Human Ciliated Epithelial Cells

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Abstract

The process and regulation of ciliogenesis in human epithelia is little understood and many components of the cilium and associated structures have not been characterised. We have identified a monoclonal antibody, LhS28, which recognises a 44,000–45,000 Mr protein specifically associated with human ciliated epithelial cells. Immunoperoxidase labelling of formalin-fixed paraffin wax-embedded human tissues showed that LhS28 was expressed in the sub-apical zone of ciliated epithelial cells of the Fallopian tube and upper respiratory tract, but not ciliated ependyma, non-ciliated epithelia or testis containing developing spermatozoa. Immunoelectron microscopy demonstrated that the antigen recognised by LhS28 was associated with the basal body structure of the cilium and specifically with the 9+0 microtubule arrays. LhS28 should be a useful tool in the identification of ciliated cells in pathological specimens and for investigating mechanisms of ciliogenesis.

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Comer, M.T., Shires, M., Goode, N.P. et al. Expression of an Antigen Associated with Basal Bodies of Human Ciliated Epithelial Cells. Histochem J 31, 39–43 (1999). https://doi.org/10.1023/A:1003470113851

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  • DOI: https://doi.org/10.1023/A:1003470113851

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