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  • Original Paper
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Rb and p130 regulate RNA polymerase I transcription: Rb disrupts the interaction between UBF and SL-1

Oncogene volume 19, pages 4988–4999 (2000)Cite this article

Abstract

We have previously demonstrated that the protein encoded by the retinoblastoma susceptibility gene (Rb) functions as a regulator of transcription by RNA polymerase I (rDNA transcription) by inhibiting UBF-mediated transcription. In the present study, we have examined the mechanism by which Rb represses UBF-dependent rDNA transcription and determined if other Rb-like proteins have similar effects. We demonstrate that authentic or recombinant UBF and Rb interact directly and this requires a functional A/B pocket. DNase footprinting and band-shift assays demonstrated that the interaction between Rb and UBF does not inhibit the binding of UBF to DNA. However, the formation of an UBF/Rb complex does block the interaction of UBF with SL-1, as indicated by using the 48 kDa subunit as a marker for SL-1. Additional evidence is presented that another pocket protein, p130 but not p107, can be found in a complex with UBF. Interestingly, the cellular content of p130 inversely correlated with the rate of rDNA transcription in two physiological systems, and overexpression of p130 inhibited rDNA transcription. These results suggest that p130 may regulate rDNA transcription in a similar manner to Rb.

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Acknowledgements

RD Hannan was supported by an American Heart Association Grant-in-Aid. This study was supported in part by National Institute of Health grants GM48991 (LI Rothblum), DK15658 (LS Jefferson), and an award from the Geisinger Foundation (LI Rothblum).

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Author notes

  1. K M Hannan

    Present address: Peter MacCallum Cancer Institute, Locked Bag 1, A'Beckett Street, Melbourne, 8006, Victoria, Australia

Authors and Affiliations

  1. Henry Hood Research Program, Weis Center for Research, Geisinger Clinic, 100 N. Academy Ave., Danville, 17822, Pennsylvania, PA, USA

    K M Hannan, R D Hannan, S D Smith, Mingyue Lun & L I Rothblum

  2. Department of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, 500 University Drive, Hershey, 17033, Pennsylvania, PA, USA

    L S Jefferson

  3. Baker Medical Research Institute, P.O. Box 6492, Melbourne, 8008, Victoria, Australia

    R D Hannan

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Hannan, K., Hannan, R., Smith, S. et al. Rb and p130 regulate RNA polymerase I transcription: Rb disrupts the interaction between UBF and SL-1. Oncogene 19, 4988–4999 (2000). https://doi.org/10.1038/sj.onc.1203875

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