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Oral cancer in vivo gene expression profiling assisted by laser capture microdissection and microarray analysis

Oncogene volume 20, pages 6196–6204 (2001)Cite this article

Abstract

Large scale gene expression profiling was carried out on laser capture microdissected (LCM) tumor and normal oral epithelial cells and analysed on high-density oligonucleotide microarrays. About 600 genes were found to be oral cancer associated. These oral cancer associated genes include oncogenes, tumor suppressors, transcription factors, xenobiotic enzymes, metastatic proteins, differentiation markers, and genes that have not been implicated in oral cancer. The database created provides a verifiable global profile of gene expression during oral carcinogenesis, revealing the potential role of known genes as well as genes that have not been previously implicated in oral cancer.

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Acknowledgements

The work is supported by the National Institute of Dental and Craniofacial Research (NIDCR) grants P01 DE12467 (DTW Wong), P30 DE11814 (DTW Wong), R29 DE11983 (R Todd), Harvard University William F. Milton Fund (R Todd), Oral & Maxillofacial Surgery Foundation (H Ohyama and R Todd), and China Site Key Basic Research Program Grant G199805123 (X Zhang). H Ohyama is supported by the Scholar in Medicine Fellowship, Harvard Medical School.

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Author notes

  1. Ilias Alevizos and Mamatha Mahadevappa: I Alevizos and M Mahadevappa contributed equally and should be considered as first author

Authors and Affiliations

  1. Department of Oral Medicine and Diagnostic Sciences, Division of Oral Pathology, 188 Longwood Avenue, Harvard School of Dental Medicine, Boston, MA 02115, Massachusetts, USA

    Ilias Alevizos, Hiroe Ohyama, Yohko Kohno, George T Gallagher, Dae Kim & David T W Wong

  2. Affymetrix Inc., 3380 Central Expressway, Santa Clara, CA 95051, California, USA

    Mamatha Mahadevappa & Janet A Warrington

  3. Department of Biology, China Medical University, Shenyang, 110001, China

    Xue Zhang

  4. Department of Medicine, Division of Hematology Oncology, Dana Farber Cancer Institute, Boston, MA 02115, Massachusetts, USA

    Marshall Posner

  5. Department of Otolaryngology, Massachusetts Eye & Ear Infirmary, Boston, MA 02115, Massachusetts, USA

    Mark Varvares

  6. University of Nebraska Medical Center, College of Dentistry, Lincoln, NE 68583-0740, Nebraska, USA

    Donald Cohen

  7. The Forsyth Institute, Boston, MA 02115, Massachusetts, USA

    Ralph Kent

  8. Department of Oral and Maxillofacial Surgery, Massachusetts General Hospital, Boston, MA 02114, Massachusetts, USA

    R Bruce Donoff & Randy Todd

  9. Chung Shan Medical and Dental College, Taichung, Taiwan

    Chou Ming Yung

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  1. Ilias Alevizos
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  16. David T W Wong
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Corresponding author

Correspondence to David T W Wong.

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Alevizos, I., Mahadevappa, M., Zhang, X. et al. Oral cancer in vivo gene expression profiling assisted by laser capture microdissection and microarray analysis. Oncogene 20, 6196–6204 (2001). https://doi.org/10.1038/sj.onc.1204685

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