Gastroenterology

Gastroenterology

Volume 113, Issue 4, October 1997, Pages 1099-1109
Gastroenterology

Helicobacter pylori infection activates NF-kappa B in gastric epithelial cells

https://doi.org/10.1053/gast.1997.v113.pm9322504Get rights and content

Abstract

BACKGROUND & AIMS: Helicobacter pylori adheres to gastric epithelial cells and stimulates interleukin (IL)-8 production. This may be instrumental in neutrophil infiltration of the gastric epithelium that characterizes H. pylori gastritis. This study examined the molecular mechanisms leading to H. pylori-induced epithelial cell IL-8 production.

METHODS: Electrophoretic mobility shift analyses for NF- kappa B were performed on cell and nuclear extracts from H. pylori- infected AGS and Kato III human gastric epithelial cells.

RESULTS: H. pylori infection activated the transcription factor NF-kappa B and induced nuclear translocation of both NF-kappa B p50/p65 heterodimers and p50 homodimers. Nuclear translocation of NF-kappa B (30 minutes) was followed by increased IL-8 messenger RNA (1 hour) and protein levels (4 hours) consistent with NF-kappa B up-regulation of IL-8 gene transcription. Pretreatment of AGS cells with PDTC, which blocks NF- kappa B activation, inhibited H. pylori-induced increases in IL-8 production by 90%. Immunohistochemical studies using a monoclonal antibody that recognizes the I-kappa B binding region of p65 showed activated NF-kappa B in gastric epithelial cells of patients with H. pylori gastritis.

CONCLUSIONS: H. pylori infection activates NF-kappa B in gastric epithelial cells in vitro and in vivo. NF-kappa B is a transcriptional regulator of IL-8 production, and its activation after bacterial infection may be an important defense response in gastrointestinal epithelial cells.

(Gastroenterology 1997 Oct;113(4):1099-109)

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