Elsevier

Human Pathology

Volume 33, Issue 8, August 2002, Pages 806-811
Human Pathology

Original Contributions
Expression of CD10 by stromal cells during colorectal tumor development*,**

https://doi.org/10.1053/hupa.2002.125773Get rights and content

Abstract

CD10 is a cell surface metalloprotease expressed by a variety of normal cell types, including lymphoid precursor cells, germinal center B lymphocytes, and some epithelial cells. We noticed that stromal cells of some cancers are positive for CD10. In this study, we investigated the role of CD10 produced by the stromal cells of colorectal neoplasms in the progression of colorectal neoplasms. Immunohistochemical examination of CD10 and p53 was performed in 169 colorectal epithelial neoplasms representing various stages of carcinogenesis. The results were correlated with the morphologic characteristics of the neoplasms. There was no expression of CD10 in the stromal cells of normal colorectal tissue. CD10-positive stromal cells were present adjacent to the tumor cells in 16 of 73 adenomas with mild or moderate dysplasia. More frequent expression of CD10 by the stromal cells was detected in adenomas with severe dysplasia (12 of 17), intramucosal carcinomas (10 of 16), and invasive carcinomas (50 of 63) than in adenomas with mild or moderate dysplasia (P < 0.0001). Expression of CD10 by > 10% of the stromal cells was detected only within the area of the invasive growth front of invasive carcinomas, not in adenomas and in only 1 of the intramucosal carcinomas. The difference between invasive and non invasive tumors was significant (P < 0.0001). The stromal expression of CD10 was significantly associated with the accumulation of p53 and a larger tumor size. These results indicate that CD10 expression is an integral part of colorectal carcinogenesis. CD10 expression seems to contribute to the invasion and thus probably facilitates metastasis. HUM PATHOL 33:806-811. Copyright 2002, Elsevier Science (USA). All rights reserved.

Section snippets

Patients and tumor specimens

The subjects of this study were 169 Japanese patients who underwent colectomy at Tokyo Medical University Hospital, with 10 normal colorectal tissue samples used as controls. The blocks of formalin-fixed, paraffin-embedded tissue from these patients were retrieved from the Diagnostic Pathology Division. All pathologic diagnoses were confirmed independently by 3 pathologists reviewing hematoxylin and eosin (H&E)-stained sections. Briefly, adenomas were divided into 3 grades of dysplasia

CD10 expression in the stromal cells

Expression of CD10 by the tumor cells was not examined in this study. There was no expression of CD10 in the stromal cells of the 10 normal colorectal tissue samples. CD10 was positive in 88 of 169 colorectal tumors (52.1%). CD10-positive stromal cells appeared adjacent to the tumor cells and surrounded the neoplastic tubules or glands in 16 of 73 adenomas (21.9%) with mild or moderate dysplasia. Stromal CD10 was positive in 12 of 17 adenomas (70.6%) with severe dysplasia, in 10 of 16

Discussion

This study demonstrated that CD10 is frequently expressed by the stromal cells within adenomas with severe dysplasia grade and carcinomas. CD10 is expressed by the tumor cells in different organs13, 14, 15 and is associated with differentiation and growth of tumor cells.16, 17 Few studies have demonstrated CD10 expression by tumor-associated stromal cells. Ours is the first study that has investigated CD10 expression by the stromal cells within colorectal tumors. According to the theory of the

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    *

    Supported in part by Grants in Aid for Cancer Research and for the second-term Comprehensive Strategy for Cancer Control from the Ministry of Health, Labour, and Welfare.

    **

    Address correspondence and reprint requests to Kiyoshi Mukai, MD, Department of Pathology, Tokyo Medical University, Shinjuku 6-1-1, Shinjuku-ku, Tokyo, 160-8402 Japan.

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