Original ContributionsCTNNB1 mutations and β-catenin expression in endometrial carcinomas*,**
Section snippets
Tissue samples and DNA isolation
Paired tumor and normal samples from 73 consecutive patients with endometrial carcinomas were retrieved from the surgical pathology files of the Department of Pathology, Hospital Santa Creu i Sant Pau, Barcelona, Spain. Clinical information, including follow-up data, pathology reports, paraffin blocks, and slides, were available for review in all cases. All tumors were classified using the World Health Organization (WHO) criteria. Tumors were surgically staged and graded according to the
Pathological results
Fifty-nine tumors were endometrioid adenocarcinomas, and the remaining 14 were nonendometrioid carcinomas (5 papillary serous, 3 clear cell, 2 mixed papillary serous and clear cell, and 4 papillary serous with a carcinosarcoma component). Twenty-one endometrioid carcinomas were FIGO grade I, 21 were grade II, and 17 were grade III. All nonendometrioid carcinomas were high-grade tumors. Twenty-six tumors invaded less than 50% of the myometrial wall, whereas deep myometrial invasion was found in
Discussion
The gene that encodes β-catenin, CTNNB1, maps to 3p21. β-catenin is involved in the organization of tissue architecture and cell polarity and also plays a role in transcriptional activation. Cytoplasmic levels of β-catenin are regulated by the opposing actions of Wnt-1 and APC. Wnt-1 protein is a secreted growth factor that stimulates the accumulation of β-catenin, which can be incorporated into the E-cadherin-catenin unit together with E-cadherin, α-catenin, and γ-catenin in connection with
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2021, Translational OncologyCitation Excerpt :Since CTNNB1 mutations seemingly occur in atypical endometrial hyperplasia, CTTNB1 mutation plays a critical role in the initiation and early progression of EEC tumors [34]. Other studies reported an increase in CTNNB1 mutations in EEC compared to NEEC [35,36], and no CTNNB1 mutation was observed in the NEEC tumors in the present study. CTNNB1 mutation was detected in 25.6% (21/82) of the EEC tumors, which was similar to the result of previous reports [35–37].
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Supported by Grants FIS 99/1145 and FIS 01/1656 and by the Asociacion Española Contra el Cancer, Spain.
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Address correspondence and reprint requests to Xavier Matias-Guiu, MD, Department of Pathology, Hospital de la Santa Creu i Sant Pau, Avda Sant Antoni Ma Claret 167, 08025 Barcelona, Spain.