Application of short tandem repeat of genomic DNA and mitochondrial DNA for identification of mixed-up tissue specimens

Pathol Int. 2000 Jan;50(1):1-6. doi: 10.1046/j.1440-1827.2000.01010.x.

Abstract

Deoxyribonucleic acid (DNA) typing methods, short tandem repeat of genomic DNA and mitochondrial DNA with the use of polymerase chain reaction amplification, were applied to formalin-fixed, paraffin-embedded tissues submitted for diagnosis, to identify and sort out mixed-up tissue specimens. These techniques were found to be reliable, reproducible and specific for personal identification, and thus to eliminate the need for further examinations and to prevent unnecessary surgery.

Publication types

  • Case Reports

MeSH terms

  • Aged
  • DNA Fingerprinting / methods*
  • DNA Primers / chemistry
  • DNA, Mitochondrial / genetics*
  • DNA, Neoplasm / analysis
  • Genome*
  • Genotype
  • Humans
  • Male
  • Middle Aged
  • Polymerase Chain Reaction
  • Stomach Neoplasms / genetics*
  • Stomach Neoplasms / pathology
  • Tandem Repeat Sequences / genetics*

Substances

  • DNA Primers
  • DNA, Mitochondrial
  • DNA, Neoplasm