Abstract
To search for possible anti-tumor promoters, thirteen flavones (1-13) obtained from the peel of Citrus plants were examined for their inhibitory effects on the Epstein-Barr virus early antigen (EBV-EA) activation by a short-term in vitro assay. Of these flavones, 3,5,6,7,8,3',4'-heptamethoxyflavone (HPT) (13) exhibited significant inhibitory effects on the EBV-EA activation induced by the tumor promoter, 12-O-tetradecanoylphorbol 13-acetate (TPA). Further, compound 13 exhibited remarkable inhibitory effects on mouse skin tumor promotion in an in vivo two-stage carcinogenesis test.
MeSH terms
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Animals
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Antigens, Viral / metabolism*
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Female
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Flavonoids / chemistry
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Flavonoids / isolation & purification
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Flavonoids / pharmacology*
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Mice
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Mice, Inbred ICR
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Papilloma / chemically induced
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Papilloma / drug therapy
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Papilloma / metabolism*
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Plant Extracts / chemistry
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Plant Extracts / isolation & purification
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Plant Extracts / pharmacology*
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Skin Neoplasms / chemically induced
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Skin Neoplasms / drug therapy
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Skin Neoplasms / metabolism*
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Tetradecanoylphorbol Acetate
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Time Factors
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Virus Activation / drug effects*
Substances
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Antigens, Viral
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Epstein-Barr virus early antigen
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Flavonoids
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Plant Extracts
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3,3',4',5,6,7,8-heptamethoxyflavone
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Tetradecanoylphorbol Acetate