Long-term preservation of strains of Burkholderia cepacia, Pseudomonas spp. and Stenotrophomonas maltophilia isolated from patients with cystic fibrosis

Lett Appl Microbiol. 2001 Jul;33(1):82-3. doi: 10.1046/j.1472-765x.2001.00947.x.

Abstract

Long-term preservation methods are important in the maintenance of bacteria for downstream research applications. Most clinical laboratories have only limited resources for archiving isolates and therefore require cost-effective and simple methods. An effective and cheap storage method using debrinated blood and maintenance at -80 degrees C is described.

MeSH terms

  • Bacteriological Techniques*
  • Burkholderia cepacia* / physiology
  • Cryopreservation*
  • Cystic Fibrosis / microbiology*
  • Humans
  • Pseudomonas* / physiology
  • Specimen Handling
  • Stenotrophomonas maltophilia*
  • Time Factors