Abstract
Pyruvate kinase type M(2) from Morris hepatoma 7777 tumour cell nuclei and cytosol, in contrast to types L and M(2) from nuclei and cytosol of normal rat liver, shows the histone H(1) kinase activity. Moreover, in the presence of L-cysteine and without ADP it converts 2-phosphoenolpyruvate (PEP) to pyruvate while in the presence of L-arginine or L-histidine does not. L-Cysteine markedly stimulates the activity of histone H(1) kinase transferring a phosphate group from PEP to, as results suggested, the epsilon -amino group of L-lysine of histone H(1). This, L-cysteine which is known to inhibit the activity of pyruvate kinase type M(2) from neoplastic cells transfering a phosphate from PEP to ADP, can act as a control factor champing the direction of enzymatic reaction in cancer cells.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine Triphosphate / pharmacology
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Animals
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Arginine / pharmacology
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Cell Fractionation
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Chromatin / isolation & purification
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Chromatin / metabolism
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Cysteine / pharmacology
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Cytosol / enzymology
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Electrophoresis, Polyacrylamide Gel
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Enzyme Inhibitors / pharmacology
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Histidine / pharmacology
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Isoenzymes / chemistry
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Isoenzymes / drug effects
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Isoenzymes / metabolism
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Liver / enzymology*
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Liver Neoplasms, Experimental / enzymology*
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Phosphoenolpyruvate / pharmacology
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Pyruvate Kinase / chemistry
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Pyruvate Kinase / drug effects
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Pyruvate Kinase / metabolism*
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Rats
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Rats, Inbred BUF
Substances
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Chromatin
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Enzyme Inhibitors
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Isoenzymes
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Histidine
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Phosphoenolpyruvate
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Adenosine Triphosphate
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Arginine
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Pyruvate Kinase
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Cysteine