Abstract
Fourier transform infrared microspectroscopy (FTIR-MSP), which is based on the characteristic molecular vibrational spectra of cells, was used to investigate spectral differences between normal primary rabbit bone marrow (BM) cells and bone marrow cells transformed (BMT) by murine sarcoma virus (MuSV). Primary cells, rather than cell lines, were used for this research because primary cells are similar to normal tissue cells in most of their characteristics. Our results showed dramatic changes in absorbance between the control cells and MuSV124-transformed cells. Various biological markers, such as the phosphate level and the RNA/DNA obtained, based on the analysis of the FTIR-MSP spectra, also displayed significant differences between the control and transformed cells. Preliminary results suggested that the cluster analysis performed on the FTIR-MSP spectra yielded 100% accuracy in classifying both types of cells.
Publication types
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Comparative Study
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amides / analysis
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Amides / chemistry
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Amides / metabolism
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Animals
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Biomarkers, Tumor / analysis
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Biomarkers, Tumor / chemistry
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Biomarkers, Tumor / metabolism*
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Bone Marrow Cells / chemistry
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Bone Marrow Cells / classification
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Bone Marrow Cells / metabolism*
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Bone Marrow Cells / virology
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Cell Transformation, Viral
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Cluster Analysis
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DNA, Neoplasm / analysis
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DNA, Neoplasm / chemistry
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DNA, Neoplasm / metabolism
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Fibroblasts / chemistry
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Fibroblasts / classification
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Fibroblasts / metabolism*
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Fibroblasts / pathology
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Fibroblasts / virology
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Mice
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Microspectrophotometry / methods*
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Phosphates / analysis
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Phosphates / chemistry
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Phosphates / metabolism
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RNA, Neoplasm / analysis
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RNA, Neoplasm / chemistry
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RNA, Neoplasm / metabolism
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Rabbits
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Reference Values
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Sarcoma Viruses, Murine
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Spectroscopy, Fourier Transform Infrared / methods*
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Tumor Cells, Cultured
Substances
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Amides
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Biomarkers, Tumor
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DNA, Neoplasm
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Phosphates
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RNA, Neoplasm