Nanopore-based fourth-generation DNA sequencing technology

Yanxiao Feng; Yuechuan Zhang; Cuifeng Ying; Deqiang Wang; Chunlei Du

doi:10.1016/j.gpb.2015.01.009

Nanopore-based fourth-generation DNA sequencing technology

Genomics Proteomics Bioinformatics. 2015 Feb;13(1):4-16. doi: 10.1016/j.gpb.2015.01.009. Epub 2015 Mar 2.

Authors

Yanxiao Feng¹, Yuechuan Zhang², Cuifeng Ying³, Deqiang Wang⁴, Chunlei Du¹

Affiliations

¹ Chongqing Key Laboratory of Multi-scale Manufacturing Technology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, China; University of Chinese Academy of Sciences, Beijing 100049, China.
² Chongqing Key Laboratory of Multi-scale Manufacturing Technology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, China; School of Physical Electronics, University of Electronic Science and Technology of China, Chengdu 611731, China.
³ Chongqing Key Laboratory of Multi-scale Manufacturing Technology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, China; MOE Key Laboratory of Weak-light Nonlinear Photonics, School of Physics, Nankai University, Tianjin 300071, China.
⁴ Chongqing Key Laboratory of Multi-scale Manufacturing Technology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: dqwang@cigit.ac.cn.

Abstract

Nanopore-based sequencers, as the fourth-generation DNA sequencing technology, have the potential to quickly and reliably sequence the entire human genome for less than $1000, and possibly for even less than $100. The single-molecule techniques used by this technology allow us to further study the interaction between DNA and protein, as well as between protein and protein. Nanopore analysis opens a new door to molecular biology investigation at the single-molecule scale. In this article, we have reviewed academic achievements in nanopore technology from the past as well as the latest advances, including both biological and solid-state nanopores, and discussed their recent and potential applications.

Keywords: DNA sequencing; Nanopore; Single base; Single molecule.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Genome, Human*
Humans
Nanopores*
Nanotechnology / instrumentation
Nanotechnology / methods*
Sequence Analysis, DNA / instrumentation*
Sequence Analysis, DNA / methods*