Creating low-power photomicrographs using a 35 mm digital slide scanner

Am J Surg Pathol. 1997 Jul;21(7):865-6. doi: 10.1097/00000478-199707000-00017.

Abstract

A method for obtaining high quality photomicrographs at very low power magnification using a digital slide scanner is described. Using an easily modified 35-mm film slide mount, it is possible to scan a conventional 25-mm wide glass slide and to obtain an image that is uniformly in focus and of even illumination. The computer bitmap file that is generated is suitable for computer presentations or publication-quality prints and may be magnified up to 12-fold without significant image degradation.

MeSH terms

  • Calibration
  • Equipment Design
  • Image Processing, Computer-Assisted*
  • Photomicrography / instrumentation
  • Photomicrography / methods*