Abstract
The pim-1 oncogene is regulated by hematopoietic cytokine receptors, encodes a serine/threonine protein kinase, and cooperates with c-myc in lymphoid cell transformation. Using a yeast two-hybrid screen, we found that Pim-1 protein binds to p100, a transcriptional coactivator that interacts with the c-Myb transcription factor. Pim-1 phosphorylated p100 in vitro, formed a stable complex with p100 in animal cells, and functioned downstream of Ras to stimulate c-Myb transcriptional activity in a p100-dependent manner. Thus, Pim-1 and p100 appear to be components of a novel signal transduction pathway affecting c-Myb activity, linking all three to the cytokine-regulated control of hematopoietic cell growth, differentiation, and apoptosis.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Acetyltransferases*
-
Animals
-
Base Sequence
-
Cells, Cultured
-
Endonucleases
-
Gene Expression Regulation, Enzymologic
-
Gene Expression Regulation, Neoplastic
-
Mice
-
Molecular Sequence Data
-
Nuclear Proteins / genetics
-
Nuclear Proteins / metabolism*
-
Promoter Regions, Genetic / physiology
-
Protein Serine-Threonine Kinases / genetics
-
Protein Serine-Threonine Kinases / metabolism*
-
Proteins / genetics
-
Proteins / metabolism
-
Proto-Oncogene Proteins / genetics
-
Proto-Oncogene Proteins / metabolism*
-
Proto-Oncogene Proteins c-myb
-
Proto-Oncogene Proteins c-pim-1
-
Signal Transduction / physiology*
-
Trans-Activators / genetics
-
Trans-Activators / metabolism*
-
ras Proteins / metabolism
Substances
-
Nuclear Proteins
-
Proteins
-
Proto-Oncogene Proteins
-
Proto-Oncogene Proteins c-myb
-
Trans-Activators
-
Acetyltransferases
-
mim-1 protein, Gallus gallus
-
Pim1 protein, mouse
-
Protein Serine-Threonine Kinases
-
Proto-Oncogene Proteins c-pim-1
-
Endonucleases
-
Snd1 protein, mouse
-
ras Proteins