The stability of 25 analytes from serum of healthy donors was determined at room
temperature, 4dC, and-20dC over 48 h, 14 days, and 4 months, respectively. Glucose, blood …

We examined the effects of temperature on the activity and steady-state kinetic properties of
alkaline phosphatase (EC 3.1. 3.1). Purified isoenzymes from human liver, intestine, and …

We describe our experience with Mercer's method [Clin. Chem. 20, 36 (1974)] for separating
isoenzymes of creatine kinase (EC 2.7. 3.2) in serum by ion-exchange chromatography. By …

We further modified a diethylaminoethyl-cellulose column-chromatographic system [Clin.
Chem. 21, 392 (1975)] so that it will separate all three serum creatine kinase isoenzymes …

It is our intention in this review to evaluate the diagnostic usefulness of the measurement of
serum enzymes by muss compared to the more conventional assays of enzyme activity. For …

We assessed the stability--at 20, 4--20, and--80 degrees C--of 2-mercaptoethanol,
dithioerythritol, and dithiothreitol, and of semi-purified creatine kinase isoenzymes of human …

We present a method for preparing human liver lactate dehydrogenase (L-lactate: NAD+
oxidoreductase; EC 1.1. 1.27) isoenzyme-5 by sequential ion-exchange chromatography …

In vitro incubation, at 37 degrees C, of human creatine kinase isoenzyme-1 (isoenzyme BB)
and human immunoglobulin G in a buffer results in the formation of a complex of high …

The activity of the brain specific isoenzyme of creatine kinase is shown to fall off rapidly at 37
degrees C, particularly in the presence of albumin. Dithiothreitol cannot reverse this lability …

We report the effect of serum pH on the storage stability of the human creatine kinase
isoenzymes and on the creatine kinase assay lag phase (Szasz et al., Clin. Chem. 22: 650 …